Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9 on resea.org. DOI 10.1038/nbt.3437. John G. Doench, Nicolò Fusi, Meagan E. Sullender, Mudra Hegde,…
John G. Doench, Nicolò Fusi, Meagan E. Sullender, Mudra Hegde, Emma W Vaimberg, Katherine Donovan, Ian C. P. Smith, Zuzana Tóthová